ULTRASTRUCTURAL EXAMINATION OF INTRACELLULAR CALCIUM CHANGES IN OVARIAN CANCER
FRESENIUS ENVIRONMENTAL BULLETIN, vol.26, no.11, pp.6588-6598, 2017 (SCI-Expanded)
- Publication Type: Article / Article
- Volume: 26 Issue: 11
- Publication Date: 2017
- Journal Name: FRESENIUS ENVIRONMENTAL BULLETIN
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
- Page Numbers: pp.6588-6598
- Eskisehir Osmangazi University Affiliated: Yes
Abstract
In this study, following the fibronectin-coated integrin activation in ovarian cancer cells (SKOV-3), endoplasmic reticulum (ER) stress was generated by intracellular calcium increase and then possible apoptotic effects were investigated. Tunicamycin (TN) concentrations and intracellular calcium alterations in SKOV-3 cells were analyzed by flow cytometry using Fluo-3 AM ye Rhod-2 AM flou-rescent dyes. Apoptotic cell death dependent to ER stress that generated after the treatment with 18 mu M TN were investigated by Annexin V / PI protocol and the localisations of cytochrome c and EndoG proteins were also imaged by fluorescent microscope. In addition, morphological alterations were determined by Transmission electron microscope.