ULTRASTRUCTURAL EXAMINATION OF INTRACELLULAR CALCIUM CHANGES IN OVARIAN CANCER
FRESENIUS ENVIRONMENTAL BULLETIN, cilt.26, sa.11, ss.6588-6598, 2017 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 11
- Basım Tarihi: 2017
- Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
- Sayfa Sayıları: ss.6588-6598
- Eskişehir Osmangazi Üniversitesi Adresli: Evet
Özet
In this study, following the fibronectin-coated integrin activation in ovarian cancer cells (SKOV-3), endoplasmic reticulum (ER) stress was generated by intracellular calcium increase and then possible apoptotic effects were investigated. Tunicamycin (TN) concentrations and intracellular calcium alterations in SKOV-3 cells were analyzed by flow cytometry using Fluo-3 AM ye Rhod-2 AM flou-rescent dyes. Apoptotic cell death dependent to ER stress that generated after the treatment with 18 mu M TN were investigated by Annexin V / PI protocol and the localisations of cytochrome c and EndoG proteins were also imaged by fluorescent microscope. In addition, morphological alterations were determined by Transmission electron microscope.