AGBIOL 2022, Tekirdağ, Türkiye, 28 - 31 Ağustos 2022, ss.1
Fertilization biology is very important criteria for fruitful. Pollen germination and pollen tube
growth are major components of fertilization success in fruit trees. A mature pollen is used for
the germination of pollen in vitro and in vivo in its nutrient storage and nutrient content, just
like the seed. However, the nutrients contained in the pollen are often not enough for the pollen
tubes to reach the seed drafts. For this reason, the development of pollen tubes after a certain
stage is realized through the use of nutrients in the stigma. In this study, pollens belonging to
‘Red Lake’ and Rosenthall varieties were used to examine the effects of certain growth
regulators and mineral substances on pollen germination and pollen tube growth. “Agar in
plate” method was used the for germination tests which was contain 0,5g agar-agar +15g
sucrose + 5ppm boric acid, potassium nitrate (50 ppm), thioure (50 ppm), benzyladenine
(5ppm), gibberellic acid (10 ppm) and indole butyric acid (10 ppm) solutions were added
germination medium (0.5 g agar- agar +15g sucrose + 5ppm boric acid and incubated at 21 0C
Pollen tube growth was measured by ocular micrometer after 24 hours later germination.
Statistical analyses were performed by SPSS 22.0 version. The effects of chemicals on pollen
germination and tube growth were found as statistically different. according to minerals and
plant growth regulators. Potassium nitrate and gibberellic acid were determined as promoter
while thioure and benzyladenine effected as inhibitory in pollen germination and tube growth.