Selenium in the treatment of colon cancer: Investigation of effect on epithelial mesenchymal transition

---

Güneş Bağış S., Tomsuk Ö., Ergen F. B., Eker Sarıboyacı A., Uysal O.

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, cilt.11, sa.2, ss.103-111, 2025 (Hakemli Dergi)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 11 Sayı: 2
• Basım Tarihi: 2025
• Doi Numarası: 10.30569/adiyamansaglik.1694274
• Dergi Adı: Adıyaman Üniversitesi Sağlık Bilimleri Dergisi
• Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
• Sayfa Sayıları: ss.103-111
• Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Aim: This study aimed to investigate the therapeutic effect of seleno-L-methionine (SLM), a selenium (Se) compound, on colorectal cancer (CRC) cells through epithelial-mesenchymal transition (EMT)-related transcription factors (ZEB1 and ZEB2).
Materials and Methods: The human colon carcinoma cell line (Caco-2) was treated with different concentrations of SLM (15.6–250 nmol/L) for 24 hours. Cell viability was assessed using the MTT assay, live/dead rate and morphology were evaluated by Calcein-AM/Propidium Iodide staining, and ZEB1 and ZEB2 gene expressions were analyzed by qRT-PCR.
Results: SLM decreased cell viability in a dose-dependent manner (IC50: 31.25 nmol/L). Live/dead analysis revealed a significant increase in non-viable cells in the SLM-treated group compared to controls, although clear apoptotic morphology was not consistently observed. qRT-PCR analysis revealed a significant downregulation of ZEB2 and a slight reduction in ZEB1 expression.
Conclusion: SLM exhibited cytotoxic effects and modulated EMT-related gene expression, suggesting potential therapeutic implications in CRC.