Essential oil and lipids from leaves of Ferula kuhistanica Korovin (Apiaceae; syn. F. jaeschkeana auct. non Vatke) growing in Uzbekistan were studied for the first time using GC-MS. Essential oil consisted of 51 constituents (99.8% total with mostly alpha-pinene, 72.9%). Enantioselective analysis of essential-oil constituents found the enantiomeric excesses of (+/-)-alpha-pinene (61.9/38.1), (+/-)-beta-pinene (28.6/71.4), (+/-)-sabinene (13.2/86.8), and (+/-)-limonene (82.7/17.3). Lipids included hexadeca-7Z,10Z,13Z-trienoic acid, confirming that F. kuhistanica belonged to plant group 16:3. Essential oil, chiral constituents, and lipids from leaves of F. kuhistanica were studied for the first time.