An essential oil obtained from the crushed seeds of Matthiola anchoniifolia Hub.-Mor. (Cruciferae) was obtained by microdistillation and analyzed by both GC and GUMS. Octyl acetate (21.2%) was identified as the main constituent in the oil. Sulfur compounds responsible for the foul odor of the oil comprised 34.7%. They consisted of isopropyl isothiocyanate (16.9%), dimethyl trisulfide (8.8%), dimethyl disulfide (4.7%) and isobutyl isothiocyanate (4.3%).