Direct photoconjugation multi-wall carbon nanotube-based immunosensor platform for clenbuterol determination
FOOD CHEMISTRY, cilt.502, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 502
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.foodchem.2025.147693
- Dergi Adı: FOOD CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE
- Anahtar Kelimeler: ANADOLUCA conjugation, Clenbuterol, Immunosensor, Multi-walled carbon nanotube, Potentiometric sensor
- Eskişehir Osmangazi Üniversitesi Adresli: Evet
Özet
This study reports the development of a carbon nanotube-based potentiometric sensor for the selective and costeffective detection of clenbuterol. The sensor design depends on specific molecular recognition between clenbuterol and an anti-clenbuterol antibody covalently immobilized onto a photosensitive amino acid-modified multi-walled carbon nanotube (MWCNT) platform. This functional nanomaterial enabled the direct conjugation of the antibody-crosslinked electrode surface. An anti-clenbuterol antibody-bound MWCNT was used as the indicator electrode. An Ag/AgCl electrode was used as the reference electrode. The immunopotentiometric sensor exhibited a rapid response (1 min), a broad linear detection range from 1.0 x 10-11 M to 1.0 x 10-5 M, and an excellent low detection limit of 4.83 x 10-12 M. The operational lifetime was extended to 36 weeks, with pH 6.0 identified as the optimal working condition. The proposed immunopotentiometric sensor offers high selectivity, low detection limits, cost-effectiveness, and label-free detection, making it a promising tool for clenbuterol monitoring.