Fisetin effects on cell proliferation and apoptosis in glioma cells

Pak F., Oztopcu-Vatan P.

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, cilt.74, ss.295-302, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 74
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1515/znc-2019-0098
  • Dergi Adı: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.295-302
  • Anahtar Kelimeler: cytotoxicity, fisetin, glioma, RT-PCR, TEM, CYCLE ARREST, CANCER-CELLS, ACTIVATION, EXPRESSION, FLAVONOIDS, INHIBITION, HESPERETIN, AUTOPHAGY, CASPASE-3, WOGONIN
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

This research investigated the antiproliferative effects of 1-500 mu M fisetin in T98G and BEAS-2B cells by MIT assay. The IC50 of fisetin in T98G cells for 24 and 48 h were 93 and 75 mu M, respectively. Apoptotic alterations of fisetin-treated T98G cells were observed by transmission electron microscopy. BEAS-2B was then used in comparison to T98G cells to determine the cytotoxic effects of fisetin. The IC50 of fisetin for 24 and 48 h were recorded as 270 and 90 mu M in BEAS-2B cells, respectively. Different concentrations of fisetin were selected to determine the apoptotic and necrotic effects. Consequently, fisetin was determined to have more apoptotic effects in T98G than BEAS-2B cells, dose- and time-dependently. Moreover, fisetin was found to have cytotoxicity at lower doses in T98G cells compared to carmustine, as positive control. CASPASE3, CASPASE9, CASPASE8, and BAX expressions were increased by the selected fisetin doses of 25 and 50 mu M, while that of BCL-2 and survivin was reduced in T98G cells. These results will serve as an essential basis of future in vitro and in vivo studies, in the continuous search for alternative treatment agents for gliomas.