miRNA-146a, miRNA-155 and JNK expression levels in peripheral blood mononuclear cells according to grade of knee osteoarthritis

Soyocak A., KURT H., ÖZGEN M., TURGUT COŞAN D., ÇOLAK E., Gunes H. V.

GENE, cilt.627, ss.207-211, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 627
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.gene.2017.06.027
  • Dergi Adı: GENE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.207-211
  • Anahtar Kelimeler: Osteoarthritis, miRNA-146a, miRNA-155, JNK, PBMC, N-TERMINAL KINASE, SIGNAL-TRANSDUCTION, MICRORNAS, CARTILAGE, PATHOGENESIS, INFLAMMATION, ACTIVATION, MEDIATORS, TARGETS, PATHWAY
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Osteoarthritis (OA) is the most common joint disease characterized by joint pain and a progressive loss of articular cartilage. OA known as a non-inflammatory disease. Despite this the recent studies are shown synovitis and low inflammation to have a role in OA pathophysiology. The aim of this study to determine the roles of a potential therapeutic targets miRNA-146a, miRNA-155 and JNK expression levels in OA patients. Peripheral mononuclear blood cells (PBMCs) were extracted from OA patients and healthy subjects. The expression levels of miRNA-146a, miRNA-155 and JNK were quantified using by real-time PCR assay. According to study results a statistically significant increase was observed only in miRNA-155 expression level (p = 0,039). However, miRNA-146a and miRNA-155 expressions increased in the progressive stages (grade 3 and grade 4) in OA patients. Our data suggests that correlation of miRNAs regulating and signal pathways can play an important role in OA pathogenesis and disease progression.