The comparative effect of exogenous glutathione with nifedipine and nicardipine on H 2O 2-induced oxidation of bovine lens epithelial cells


Basmak H., Erdal Kabadere T., KABADERE S., Kus G.

Fresenius Environmental Bulletin, vol.21, no.2, pp.228-232, 2012 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 2
  • Publication Date: 2012
  • Journal Name: Fresenius Environmental Bulletin
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.228-232
  • Eskisehir Osmangazi University Affiliated: Yes

Abstract

Oxidation causes cataract formation in human and hydrogen peroxide (H 2O 2), present in aqueous humor, rises 5-10 fold and is cytotoxic in cataract patients. Lens has a high concentration of glutathione (GSH) which can detoxify the oxidants, but GSH decreases with age and development of cataract. Calcium channel blockers such as nifedipine and nicardipine display antioxidant activity. Therefore, we compared the effects of GSH with nifedipine and nicardipine on H 2O 2-induced oxidation of bovine lens epithelial cells (BLEC). BLEC were cultured in a humidified atmosphere of 5% CO 2, at 37 °C. For determination of IC 50, 100, 200, 300 or 500 μM H 2O 2 were applied for 3 hours. Simultaneously 200 μM H 2O 2 either with GSH, nicardipine (1,3 μM) or nifedipine (1,3 μM) were added to the culture medium for 3 hours, removed and refed for 24 hours. After 27 hours, drug cytotoxicity was determined by using a colorimetric assay. H 2O 2 at 200 μM induced approximately 50% cell death (p<0.001) and 1000 μM GSH was protective against H 2O 2 toxicity. GSH increased cell viability by 25% (p<0.001), but nifedipine and nicardipine did not prevent H 2O 2-caused death. When applied simultaneously with H 2O 2, GSH has a strong antioxidant effect however, nicardipine and nifedipine can not block the oxidation. © by PSP.