Effects of Simvastatin on Experimental Neuropathic Pain Model and the Role of Nitric Oxide

Aydın Ş., Yıldırım E., Erol K.

Journal of The Analgesics, no.1, pp.1-8, 2013 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Publication Date: 2013
  • Journal Name: Journal of The Analgesics
  • Page Numbers: pp.1-8
  • Eskisehir Osmangazi University Affiliated: Yes


The aim of the present study was to evaluate putative antinociceptive effects of simvastatin and contribution of

nitric oxide to experimental neuropathic pain model in rats. 48 Male, Sprague-Dawley rats (200-250g) were divided into

seven groups. Simvastatin (10/20 mg/kg, i.p.) was administered intraperitoneally for ten days to rats which were injured

by main root ligation of sciatic nerve in chronic construction injury model. The antinociceptive activity of simvastatin was

evaluated by using mechanical allodynia, thermal hyperalgesia and cold allodynia on the days of 0, 3, 7, 10. The same

tests were performed to assess the role of nitric oxide, by using simvastatin 20mg/kg with 10mg/kg L-NAME or LArginine.

Tramadol, used routinely in neuropathic pain treatment, was evaluated as a positive control. In all tests,

hindpaw withdrawal thresholds and latencies were obtained for ligated, control and sham operated groups 30 minutes

after drug or vehicle injections. Simvastatin 10/20mg/kg and tramadol 10 mg/kg groups significantly increased paw

withdrawal latency on third day in mechanical allodynia test. Simvastatin 20mg/kg and tramadol groups significantly

increased antinociceptive effect on tenth day. L-NAME+simvastatin 20mg/kg combination showed significant effect on

the tenth day. L-Arginine+simvastatin 20mg/kg significantly decreased paw withdrawal threshold as compared to

simvastatin 10/20mg/kg on the third day. There was no significant difference between groups in terms of thermal

hyperalgesia. Simvastatin 20mg/kg significantly increased paw withdrawal latency on the tenth day as compared to

control group in cold allodynia test. Simvastatin 20mg/kg significantly decreased the neuropathic pain in the present

study. As a conclusion it seems that nitric oxide pathway may contribute to antinociceptive effect of simvastatin.