In this study, glucose electrooxidation activities of carbon nanotube (CNT)-supported Pt catalysts synthesized at various weight percentages and optimum electrode preparation conditions are investigated. For glucose electrooxidation on Pt/CNT, electrode preparation parameters such as amount of catalyst ink (V-c), ultrasonication duration of the catalyst ink (d(u)), and drying duration of the electrode (d(d)) were optimized to obtain maximum specific activity. The catalysts (Pt/CNT) are characterized via N-2 adsorption-desorption, X-ray diffraction, and electron microscopy with energy dispersive X-ray advanced surface analysis techniques. Specific activity for glucose electrooxidation catalyst performance are determined by using cyclic voltammetry (CV) and chronoamperometry (CA) measurements. According to CV measurements, the best electrocatalytic activity obtained is 3.4352 mA/cm(2) with 7% Pt/CNT catalyst. Experimental conditions are optimized via response surface methodology (RSM) for maximizing glucose electrooxidation activity. The predicted specific activity and the actual specific activity are determined to be 5.931 mA/cm(2) and 5.421 mA/cm(2) under optimum conditions such as 7.36 mu L (V-c), 49.54 min (d(d)), and 2.45 min (d(u)).