Capillary electrophoretic behaviour and determination of enoxacin in pharmaceutical preparations and human serum

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Tuncel M., Dogrukol-Ak D., Senturk Z., Ozkan S., Aboul-Enein H.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, vol.24, no.16, pp.2455-2467, 2001 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 24 Issue: 16
• Publication Date: 2001
• Doi Number: 10.1081/jlc-100105951
• Journal Name: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Page Numbers: pp.2455-2467
• Eskisehir Osmangazi University Affiliated: No

Abstract

A validated capillary electrophoretic method with UV detection is described for the determination of enoxacin [ENX] in pharmaceutical preparation and human serum. The experiments were carried out in a fused-silica capillary (ID=75 tm, total 88 cm, effective 58 cm length) using a 20 mM borate buffer at pH 8.6, applying a potential of 30 kV, l s of injection. Acetylpipedimic acid was used as an internal standard (IS) and the detection was performed at 265 nm. The t(M)+/- RSD% of ENX and IS were 4.8 +/-0.9 and 5.5 +/-1.2 minutes, respectively. A well-correlated calibration equation was obtained in the range of 3.1x10(-6)-3.1x10(5) M ENX. Limit of detection (LOD) was 3.5x10(-6) M (S/N = 3).