Erythropoietin improves oxidative stress following spinal cord trauma in rats

YAZIHAN N., UZUNER K., SALMAN B., VURAL M., KÖKEN T., Arstantas A.

INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, cilt.39, sa.12, ss.1408-1413, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 12
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.injury.2008.03.010
  • Dergi Adı: INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1408-1413
  • Anahtar Kelimeler: Experimental spinal cord trauma, Erythropoietin (EPO), NMDA blocker, Ketamine, Oxidative stress, TNF-alpha, Malonyldialdehyde (MDA), Glutathione (GSH), Catalase, INJURY, DAMAGE, PROTECTS
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Spinal cord injury (SCI) is a very destructive process for both patients and society. Lipid peroxidation is the main cause of the further secondary damage which starts after mechanical destruction of tissues. Recent studies have shown that erythropoietin (EPO) has neuroprotective properties. In this study, we aimed to see the effect of EPO treatment after spinal cord injury on the oxidant and antioxidant enzyme systems and the relationship with the N-methyl-D-Aspartate (NMDA) blockage. Spinal cord injury was produced by epidural compression with a cerebral vascular clip that has a closing force of 40 g for 30 s after a limited multilevel laminectomy (T9-11). Experiment was done in 5 groups: Group1: Sham-operated untraumatised, Group 2: SCI untreated, Group 3: 150 i.u./kg EPO injected i.p. at the end of the first hour following the trauma. Group 4: NMDA receptor antagonist ketamine (100mg/kg) i.p. Group 5: EPO + ketamine i.p. The experiments were finished after 12 h of the trauma. The spinal cords were excised for biochemical examinations.