Erythropoietin improves oxidative stress following spinal cord trauma in rats


YAZIHAN N., UZUNER K., SALMAN B., VURAL M., KÖKEN T., Arstantas A.

INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, vol.39, no.12, pp.1408-1413, 2008 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 12
  • Publication Date: 2008
  • Doi Number: 10.1016/j.injury.2008.03.010
  • Journal Name: INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1408-1413
  • Keywords: Experimental spinal cord trauma, Erythropoietin (EPO), NMDA blocker, Ketamine, Oxidative stress, TNF-alpha, Malonyldialdehyde (MDA), Glutathione (GSH), Catalase, INJURY, DAMAGE, PROTECTS

Abstract

Spinal cord injury (SCI) is a very destructive process for both patients and society. Lipid peroxidation is the main cause of the further secondary damage which starts after mechanical destruction of tissues. Recent studies have shown that erythropoietin (EPO) has neuroprotective properties. In this study, we aimed to see the effect of EPO treatment after spinal cord injury on the oxidant and antioxidant enzyme systems and the relationship with the N-methyl-D-Aspartate (NMDA) blockage. Spinal cord injury was produced by epidural compression with a cerebral vascular clip that has a closing force of 40 g for 30 s after a limited multilevel laminectomy (T9-11). Experiment was done in 5 groups: Group1: Sham-operated untraumatised, Group 2: SCI untreated, Group 3: 150 i.u./kg EPO injected i.p. at the end of the first hour following the trauma. Group 4: NMDA receptor antagonist ketamine (100mg/kg) i.p. Group 5: EPO + ketamine i.p. The experiments were finished after 12 h of the trauma. The spinal cords were excised for biochemical examinations.