The copper(II) orotate complex with 2-aminoethylpyridine, [Cu(HOr)(aepy)(H2O)] center dot H2O, was synthesized and characterized by means of elemental and thermal analysis, magnetic susceptibility, IR and UV/vis spectroscopic, single crystal X-ray diffraction, and antimicrobial activity studies. The complex crystallizes in the triclinic system, space aroup P (1) over bar, and the Cu(II) ion is five-coordinate with a distorted square-pyramidal coordination geometry. The aepy ligand and the orotate dianion behave as bidentate (N,N' and N,O-acid) chelating ligands. The crystal structure is stabilized by intermolecular O-H center dot center dot center dot O and N-H center dot center dot center dot O hydrogen bonds, and the orotate ligand exhibits a double hydrogen-bonding functionality. The new compound was found active against some gram (+)/(-) bacteria and yeast Candida albicans ATCC 1023 1, but there was no activity on Aspergillus niger.