In this study, we aimed to investigate genetic differences between estivated Phassalis and hibernated
Termessos native B. terrestris populations based on 20 microsatellite loci and two mtDNA gene
regions (COI and cytb). The mean number of allele per locus, observed heterozigosity and inbreeding
coefficients were determined 12.00 and 11.00, 0.68 and 0.65, 0.22 and 0.26 in Termessos and
Phassalis populations, respectively. Pairwise FST value was calculated as 0.023 by using 20
microsatellite loci. According to the mtDNA COI gene region, all samples in both two populations
were included in a single haplotype (Haplotype B). Four different haplotypes (Haplotypes 1-4) were
determined according to the mtDNA cytb gene region. Pairwise FST value was calculated as 0.0013
according to the mtDNA cytb gene region. There were no significant differences between these two
natural B. terrestris populations in terms of the 20 microsatellite and two mtDNA loci examined.
According to results, life cycle differences of different native B. terrestris population are probably
determined by environmental factors (photoperiod, temperature, flora, moisture, altitude etc.) rather
than genetic influences.