QPCR-Guided Screening of Levansucrase: Levan Characterization and Genomic Insights

AYTAR ÇELİK P., Barut D., Altintas O. E., Enuh B. M., NURAL YAMAN B., Mutlu M. B., ...Daha Fazla

JOURNAL OF POLYMERS AND THE ENVIRONMENT, cilt.32, sa.8, ss.3654-3667, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 8
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s10924-024-03197-6
  • Dergi Adı: JOURNAL OF POLYMERS AND THE ENVIRONMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Agricultural & Environmental Science Database, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3654-3667
  • Anahtar Kelimeler: Bioactivity, Halophilic, Levan, Q-PCR
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Eighty-seven bacteria isolates were screened for the presence of the levansucrase encoding gene and those possessing the gene were used in gene expression studies and the production of levan. Based on both screening methods, isolate P19-1 was determined as the producer bacterium with the highest amounts of levan. Whole genome sequencing was done to characterize the isolate and determine its genomic potential. Both its 16 S rRNA and genome were closest to Chromohalobacter salexigens. The levansucrase gene and genes suggesting a versatile carbohydrate and nitrogen metabolism were annotated. A secondary metabolism suggestive of a possible new role in promoting plant growth in hypersaline environments was revealed. The chemical structure analysis of the produced polymer was confirmed by comparison with commercial pure Erwinia herbicola levan. Also, C. salexigens levan showed high biocompatibility. Overall, this is a practical molecular approach that will help researchers to effectively screen microbes with levan production potential.