Akademik Veri Yönetim Sistemi
Europhysiology 2018, London, İngiltere, 14 - 16 Eylül 2018, ss.418
In-vitro studies have shown that TNF-alpha significantly lower
the Epo synthesis in a dose dependent manner in HEPG2 cells
(1). Quercetin is a flavanoid found in many fruits and vegetables
such as oranges, grapefruits and onions which has
been shown to have anti-oxidant and anti-inflammatory cell
protective effect. Novel studies has shown that Quercetin
increases the amount of Epo in-vitro (2). We aimed to try to
remove the inhibition of TNF-alpha on Epo in with quercetin in
HEPG2 cells.
After detecting the non-toxic doses of TNF-alpha and Quercetin
with MTT, we seeded 5*105 HEPG2 cells in 6 well plates.
Each 6 well plates were a group and the groups were; no treatment
group (control), 10 ng/ml TNF-alpha treated group (T),
10μM/ml Quercetin treated group (Q), TNF-alpha and Quercetin
(T+Q) together treated group. After 24 hour of incubation
we collected the cells and isolated total RNA, synthesied
cDNA and performed Q-PCR for Epo. Data were analyzed by
Kruskal-Wallis test and Dunn’s test for post-hoc.
TNF-alpha significantly decreased (p<0,05) the Epo mRNA
amount according to control group. In Quercetin alone
treated group and the combination treatment group,
Epo mRNA amount were same about the control group.
More detailed studies has to be done but this initial study
shows that Quercetin may prevent the Epo suprression by
TNF-alpha. We will try to explain the mechanism to understand
the relation between Epo, TNF-alpha and Quercetin.
Quercetin may be a source of treatment for anemia in chronic
inflammatory diseases, as well as many other known effects.