Salmonella and Listeria monocytogenes are important pathogenic bacteria that responsible for food-borne diseases. Both microorganism can also contaminate food environments and they are able to form biofilms. Recently, researchers focused on antimicrobial effects of some essential oils. It is reported that the Carvacrol [2-methy1-5-(1-methylethyl) phenol] exhibited strong antimicrobial activity. In this study, we compared the effects of carvacrol on planktonic and biofilm forms of Salmonella spp and L. monocytogenes isolates. Our results showed the dose-dependent and strong antimicrobial activity of carvacrol on planktonic cells (MIK <= 0.025). Also, we demonstrated that carvacrol reduced the biofilm formation on granite surfaces by scanning electron microscope, showing that this essential oil may be an alternative for Salmonella and L. monocytogenes biofilm control at early stage in food environments. However, it has no significant effect in preformed biofilm assays except 2MIC doses at Salmonella spp. Further studies need to understand of antibiofilm effects of carvacrol.