Tannic acid decorated poly(methacrylic acid) micro and nanoparticles with controllable tannic acid release and antioxidant properties


ŞAHİNER N., BÜTÜN ŞENGEL S.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, vol.508, pp.30-38, 2016 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 508
  • Publication Date: 2016
  • Doi Number: 10.1016/j.colsurfa.2016.08.014
  • Journal Name: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.30-38
  • Keywords: Poly(methacrylic acid)-Tannic acid, Microgel/nanogels, TA conjugate, Antioxidant particles, TA decorated p(MA) colloids, HYBRID MICROGELS, NANOMATERIALS, HYPERTHERMIA, PARTICLES, CELLULOSE, HYDROGEL, MOLECULE, THERAPY, NANOGEL, CO

Abstract

Poly(methacrylic acid), p(MA), particles in the size range of nano and micrometer were prepared via emulsion and reverse suspension polymerization method, respectively. Ethylene glycol dimethacrylate (EGDMA) as a biodegradable cross-linker was used in the preparation of these p(MA) particles. Both nano and micrometer p(MA) particles were decorated with tannic acid (TA) via a direct esterification linkage upon activation of the carboxylic acid with N,N'-carbonyldiimidazole followed out by conjugation with TA to obtain p(MA)-TA. For the characterization of the particles TGA, FT-IR SEM, and light microscope were used. In vitro release of the TA from p(MA)-TA conjugates were investigated at pH 7.4 in phosphate buffered saline (PBS) by UV-vis spectrometer and result revealed that the amount of the used crosslinker has paramount significance in degradation of TA molecules. For example, p(MA)-TA microparticles with 1% of the crosslinking density released 83.24 mg TA per g particles in comparison to nanosizes p(MA)-TA particles with 10% of the crosslinking density that released 35.21 mg per g particles within four days. Additionally, antioxidant properties of all particles were evaluated by Total Phenol Content (TPC) and Trolox Equivalent Antioxidant Concentration (TEAC) methods and found that the p(MA)-TA microparticles has great antioxidant properties with TPC = 61.41 +/- 1.44 mgL(-1) gallic acid equivalent (GAE), TEAC = 248.82 +/- 1.96 mM trolox g(-1) for dry sample. (C) 2016 Elsevier B.V. All rights reserved.