The anther culture is one of the most important methods for producing doubled haploid plants and the efficiency of this method is influenced by several mentioned factors such as genotype and induction media. In this study, it was investigated that anther response of different F-2 progenies of bread wheat (Triticum aestivum L.) hybrids using MN6 and P2 induction media. The results indicated that callus production, regeneration, green and albino plant numbers are higher on MN6 media than on P2 media for all genotypes. In addition to, all investigated parameters varied between genotypes. It can be said that the response of anthers depends mainly both the genotype and media, and the most suitable induction media for obtaining doubled haploid from our wheat hybrids was MN6 medium. However, it may be needed to develop other culture conditions for this population to utilize it in an actual breeding program.