Appearance and phenotype of murine follicular dendritic cells expressing VCAM-1


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Balogh P., AYDAR Y., Tew J., Szakal A.

ANATOMICAL RECORD, vol.268, no.2, pp.160-168, 2002 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 268 Issue: 2
  • Publication Date: 2002
  • Doi Number: 10.1002/ar.10148
  • Journal Name: ANATOMICAL RECORD
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.160-168

Abstract

The architecture of lymphoid follicles is determined by a series of interactions between lymphoid and follicular stromal cells. A cardinal population in the non-lymphoid compartment is the follicular dendritic cell (FDC), whose communication with resting and activated B cells involves various adhesive interactions. The FDC phenotype variably includes the display of vascular cell adhesion molecule (VCAM-1). In this report we investigated the appearance and follicular tissue distribution of VCAM-1 in murine peripheral lymphoid tissues, and compared VCAM-1 with other FDC markers using inummohistochemistry. Correlating the appearance of VCAM-1 with other murine FDC-associated markers (CR1.2 [complement receptor 1.2 or CD35/21] and FDC-M1) revealed that the display of VCAM-1 is restricted to a subset of CR1.2-positive FDCs. We found that the expression of VCAM-1 antigen in the spleen or peripheral lymph nodes on FDCs requires antigenic stimulus, and that it coincides with germinal center formation. The VCAM-1 expression is associated with the appearance of mucosal addressin cell adhesion molecule (MAdCAM-1), with some slight differences in occurrence. The appearance of VCAM-1 and MAdCAM-1 antigens on FDCs may serve as indicators of FDC activation.