Comparative Evaluation of Transgenic and Non Transgenic Tobacco for the Phytoextraction of Nickel-Contaminated Soils

DAĞHAN H., KÖLELİ N.

EKOLOJI, cilt.21, sa.84, ss.90-97, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 84
  • Basım Tarihi: 2012
  • Doi Numarası: 10.5053/ekoloji.2012.8410
  • Dergi Adı: EKOLOJI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.90-97
  • Anahtar Kelimeler: Metallothionein, nickel, phytoextraction, transgenic tobacco, HEAVY-METALS, GLUTATHIONE, TOLERANCE, TOXICITY, PLANTS, PHYTOREMEDIATION, HYPERACCUMULATOR, ASCORBATE, GROWTH, NI2+
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Nickel (Ni) accumulation ability and phytoextraction potential of Nicotiana tabacum L. cv Petit Havana SR-1 and Metallothionein II (MTII) gene transferred transgenic plants (p-S-ScMTII) of the same cultivar were grown in hydroponic culture containing 0, 5 and 10 mg L-1 Ni for 14 days. The effects of increasing Ni concentrations on plant dry matter production, Ni accumulation, chlorophyll and glutathione concentration were investigated. Moderate wilting and wrinkling were observed upon Ni treatments at the early stage of the growth, these symptoms severely increased at the harvest in both plant types. In addition, poor root growth and dark brown colour were observed. Dry matter content of transgenic tobacco was lower than that of the non-transgenic one. With the increasing Ni doses, Ni concentration, chlorophyll level and -SH (glutathione) concentration increased. Results showed that transgenic tobacco (128 mg kg(-1)) accumulated about 1.47-fold more Ni than that of non-transgenic one in shoots (87 mg kg-1) at 10 mg L-1 Ni treatment. Consequently, transfering the MTII gene into the plants to be used for phytoextraction may be regarded as promissing in improving Ni accumulation ability of plants.