Aim: This study aimed to retrospectively investigate the newborns who were operated due to midline spinal closure defect. Material and Method: 62 newborns who were operated in our clinic between 2007-2014 were evaluated in this study. Accompanying cranial and spinal pathologies were examined by imaging methods. The timing (during first 3 days, after first 3 days) and the method (primary, secondary) of the surgery were determined. In addition, the shunt infection rate was also evaluated in the follow-up period of the patients who underwent VP shunt placement due to hydrocephalus. Results: A total of 62 newborns were included in the study; 38 (61.2%) were female and 24 (38.8%) were male. The mean follow-up time was 25 +/- 6.9 months. The midline defect was localized in the cervical (n:1, 1.6%), thoracal (n:7, 11.9%), and lumbosacral regions (n:54, 87.1%). 21 of the newborns were operated within the first 3 days after birth and 41 of the newborns were operated after the first 3 days. 8 (12.9%) patients were operated due to meningocele and 54 (87.1%) patients were operated due to myelomeningocele. 1 of 8 patients with meningocele and 48 of 54 patients with myelomeningocele underwent VP shunt placement due to hydrocephalus. 9 (14.5%) skin defects were closed secondarily and 53 (85.5%) of skin defects were closed primarily. Shunt infection was detected in 13 (21%) patients. Discussion: The most common intracranial pathology accompanying midline closure defect was hydrocephalus and the most common spinal pathologies accompanying midline closure defect were syringomyelia and tethered cord. Shunt infection rate was lower in the newborns who were operated within the first 3 days after birth. Primary or secondary closure of skin defect did not affect the shunt infection rate.