Determination of phenolic acids by a modified HPLC: Its application to various plant materials

Ozturk N., Tuncel M., Tuncel N. B.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.30, sa.4, ss.587-596, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Konu: 4
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/10826070601093911
  • Sayfa Sayıları: ss.587-596


The determination of certain phenolic acids employing a gradient HPLC method is described, in this study. The analysis was performed by using a gradient program with the two solvents system (A: methanol:water:formic acid (10:88:2 v/v); B: methanol: water: formic acid (90:8:2 v/v)). The flow rate of 1 mL (.) min(-1), injection volume of 10 mL was used and signals were detected at 280 nm. Propylparaben was a suitable compound as an internal standard for this system. The method was validated and highly repeatable (between RSD values of 0.35-1.65) and linear results were obtained. The LOD and LOQ values of the phenolic acids are in the range of (2.58 x 10(-6) 29.69 x 10(-6) M) and (7.83 x 10(-6) - 22.93 x 10(-5) M), respectively.