Characterization of Szovitsia callicarpa Volatile Constituents Obtained by Micro- and Hydrodistillation


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Demirci B., KÜÇÜKBOYACI N., ADIGÜZEL N., BAŞER K. H. C. , DEMİRCİ F.

NATURAL PRODUCT COMMUNICATIONS, cilt.5, sa.2, ss.297-300, 2010 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Konu: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1177/1934578x1000500226
  • Dergi Adı: NATURAL PRODUCT COMMUNICATIONS
  • Sayfa Sayıları: ss.297-300

Özet

The volatile constituents of Szovitsia callicarpa Fisch. & C. A. Mey. were obtained from the fruits by microdistillation, due to the limited plant material availability. Initial GC-MS analyses of the obtained material showed an unidentified major constituent with a molecular ion peak at M+ 280. After hydrodistillation of the plant material, the resulting essential oil was also analyzed by GC and GC-MS, simultaneously. In total, twenty-two compounds, representing 98.6% of the microdistilled sample, were identified, whereas seventeen components were detected in the hydrodistilled oil, representing 98.5% of the total. The GC-MS analyses showed that the samples contained an unidentified major constituent, which was further purified from the hydrodistilled essential oil by micro-column chromatography. The structure was elucidated as alpha-kessyl acetate using H-1- and C-13-NMR spectroscopic and mass spectroscopic techniques. Other major constituents in the analytes were determined as longipinene, longicyclene and kessane. In addition, both samples, as well as the major compound alpha-kessyl acetate, were tested at 1 mg/mL concentration against the pathogen Candida tropicalis and for its free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) by TLC-bioauthographic techniques. Both preliminary assays at the tested concentration showed no activity.