Akademik Veri Yönetim Sistemi
Methods in Microbiology, cilt.57, ss.235-256, 2025 (Scopus)
Species-specific primer design is a vital step in molecular biology using PCR-detection methods for the determination and identification of environmental microbes and extremophiles. There are some properties considered in the design of primer pairs in these studies. They are primer length, GC content, melting temperature, and specificity, which are significant for PCR amplifications. Many different approaches and internet tools can be used to create the primers, which are species-specific, genus-specific primers being used in environmental microbiology, microbial ecology, and bio surveillance. The findings amply demonstrate the need for better primer design to enable more accurate and quick bacterial identification This study examines species-specific primer design fundamentals, focusing on length, GC concentration, and melting temperature, which determine primer efficiency. The paper provides bacteria-specific and genus-specific primer design criteria for extremophile and environmental microbiology detection. However, primer design and/or primers improve microbial identification, biosafety surveillance, and environmental microbiology, synthetic biology, and biotechnology applications.