EXTREMELY THERMOSTABLE, EDTA-RESISTANT ALKALINE PROTEASE FROM A THERMOPHILIC GEOBACILLUS SUBTERRANEUS C2-1 ISOLATE

Akkir E. Y., Sahin Y., Gedikli S., Aytar Çelik P., Çabuk A.

JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES, cilt.7, sa.1, ss.50-56, 2017 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 1
  • Basım Tarihi: 2017
  • Doi Numarası: 10.15414/jmbfs.2017.7.1.50-56
  • Dergi Adı: JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.50-56
  • Anahtar Kelimeler: Thermostable protease, Geobacillus subterraneus, Plackett-Burman experimental design, EDTA resistance, CONIDIOBOLUS-CORONATUS, EXTREMOPHILES, OPTIMIZATION, PURIFICATION, ENZYMES
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

In this study, the alkaline protease-production capacity of a Geobacillus subterraneus C2-1 isolate from the Citgol thermal spring was investigated and optimised using a Plackett-Burman experimental design. In addition to the incubation time, which was the most important parameter, other significant factors were the peptone, glucose, and MgSO(4 center dot)7H(2)O concentrations, the activation time and the inoculum amount.