The objective of this study was to determine the performance wheat genotypes for plant height, seed yield, seed weight per spike, chlorophyll content, harvest index, protein content, sedimentation, thousand seed weight and test weight; to identify gliadin band patterns and the extent of genetic diversity; to investigate gliadin components composition and the base of gliadin polymorphisms; in bread wheat genotypes. So, significant differences between years and genotypes showed that genotypes are under genotype x environment interaction for agronomic and quality characters, and wide range of differences took place among genotypes for agronomic, quality, gliadin and glutenine electrophoretic profiles. The highest performances were taken from Altay-2000, Mufitbey and Bezostaja-1 genotypes in seed yield, seed weight per spike, plant height, chlorophyll content, thousand seed weight, test weight, harvest index, protein content and sedimentation. Similarly, These genotypes had the highest gliadin relative mobility indexes and glutenine band scores. Correlations between seed weight per spike and seed yield, chlorophyll content and seed yield were significant. Quality characteristics had significant relationships with each other. Bezostaja-1, Mufitbey and Altay-2000 were found as high yielding and quality genotypes.